Comparative Transcriptomics of Gonads Reveals the Molecular Mechanisms Underlying Gonadal Development in Giant Freshwater Prawns (Macrobrachium rosenbergii)

The giant freshwater prawn, Macrobrachium rosenbergii, is a prawn that has economic significance throughout the world. It exhibits sex-related growth dimorphism, whereby males grow significantly more rapidly than females. Therefore, study on molecular regulatory mechanism, which underlies sexual differentiation of M. both scientific and commercial importance. However, scarcity genomic transcriptomic resources severely limits our knowledge mechanisms in rosenbergii. Here, transcriptome sequencing several gonadic samples females rosenbergii was performed to investigate basis underlying gonadal development. Our results showed 2149 unigenes presented as differentially expressed genes (DEGs) ovaries compared testes males, contained 484 down-regulated 1665 up-regulated genes. Enrichment analysis DEGs revealed many these be related From analyses, confirmed by quantitative real-time PCR, male-related (Mrr, MRPINK, IR, IAGBP, TESK1, dsx) were up-regulated, female-related (ERR, Sxl3, cyclinB, Dmrt99B, PPP2A, ADCY9) also up-regulated. This indicates potential role play development Furthermore, multiple signal transduction pathways relating maturation spermatogenesis, including MAPK, identified herein. data supports previous ideas IAG IAGBP-IR signaling schemes could help regulation testis’ ERR gene regulate ovarian affecting expression ADCY9. from this provides incredibly usefully for future research practical aquaculture